Efficacy of the Green Tea, Camellia sinensis Leaves Extract on Some Biological Activities of Culex pipiens and the Detection of Its Phytochemical Constituents

Document Type : Original Article

Authors

1 Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Cairo, Egypt

2 Department of Zoology, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.

3 Department of Zoology, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt

Abstract

 The mosquitocidal and repellent activity of the green tea, Camellia sinensis leaves extract against Culex pipiens was evaluated. Toxicity of this extract increased with increasing the concentration, the recorded LC50 was 540 and 600 ppm for larvae and pupae, respectively. The extract showed a significant prolongation in both larval and pupal duration. In addition, it caused a great effect in pupation and adult emergence percent. The green tea, Camellia sinensis leaves extract was found to have a remarkable malformative effect on both larvae and pupae. The green tea leaves extract was effective in exhibiting antifeedant and repellent activity against Culex pipiens mosquitoes; it gave the highest protection (100%) from the bites of starved females at the dose of 6 mg/cm2. The major bioactive chemical constituents in green tea leaves extract were identified using HPLC and FT-IR analysis. These results suggest that the leaves extract of green tea, Camellia sinensis can be considered as alternative mosquitocide and repellent agent. 

Keywords