Biological activity of Rosa arabica (Rosaceae) Different Extracts against Rift Valley Fever Vector, Culex antennatus Becker (Diptera: Cuilicidae)

Document Type : Original Article


Zoology Department, Faculty of Science, Al-Azhar University, Cairo 11651, Egypt.


Culex antennatus Becker is widely distributed in Egypt and significantly contributes to the incidence of RVF virus in the Nile Delta. The objective of this study was to assess the efficacy of leaf extracts of Rosa arabica (methanol, n-butyl acetate, chlorobenzene, and cyclohexane) against immature stages of C. antennatus. Additionally, the study aimed to determine the impact of the tested extracts on the reproductive potential of female C. antennatus offspring produced from larvae that were treated. The maximum larval mortality (100.0 percent) was observed at concentrations of 4500, 3000, 1800, and 1600 ppm of methanol, n-butyl acetate, chlorobenzene, and cyclohexane extract, respectively, according to the results obtained. The mean length period of both larvae and pupae was significantly (P<0.05) prolonged by all examined extracts in comparison to the untreated groups. Methanolic extract derived from the leaves of R. arabica demonstrated a statistically significant impact on female fecundity across all concentrations examined. Specifically, at 3500, 3000, and 2500 ppm, fecundity increased to 170.67±1.53, 178.50±1.29, and 180.00±2.39 eggs/♀, respectively, compared to 218.90±3.38 eggs/♀ for the control group. In addition, the hatchability of eggs was significantly improved by cyclohexane extract derived from the leaves of R. arabica, particularly at the two highest concentrations (1200 and 1400 ppm), where it was 82.10 and 80.86 percent, respectively, compared to 98.50 percent for the control. The cyclohexane extract exhibited the highest efficacy against C. antennatus immature stages and females derived from treated larvae, as indicated by the obtained results. The chlorobenzene, n-butyl acetate, and methanol extracts followed suit. In general, R. arabica extracts utilized in this investigation are regarded as novel and promising agents for suppressing C. antennatus, the mosquito vector.