Applications of Inherited Sterility and Trichogramma evanescens to Control Oases Date Moth, Ephestia calidella.

Document Type : Original Article

Authors

1 Biological Applications Nuclear Dep. Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt

2 Biological Applications Dept., Nuclear Research Center, Atomic Energy Authority, Abozaabal, Egypt

3 Biological Control Researches Dep., Plant Protection Research Institute, ARC, Egypt

Abstract

Studies were conducted to appraise the effect of sub-sterilizing doses of gamma irradiation (125 and175 Gy) and egg parasitoid Trichogramma evanescenson different egg ages (1,2 and3day) of Ephestia calidellaresulting from irradiated full grow pupae male with two sub sterilizing doses(125 and175 Gy).The results revealed the first generation (F1) moths were more sterility than parent (P1) moths in the two doses applied. While their duction of hatching egg percentage in the F1 males was higher than in the F1 females in the two tested doses. Moreover, at the dose of 125 Gy, the percentages of hatched eggs were 27.7 and 33.3% in the F1 males and F1 females, respectively and decreased to 12.7and 20%in the F1 males and F1 females, respectively in the case of 175Gy. The percentage of parasitism on E. calidella eggs by T. evanescens was significantly influenced by the dose of radiation given to male parents and age of host eggs.Also, the highest rate of parasitoid eggs was occurred at 24h, while, the lowest rate was recorded at 72h old in descended of both irradiated and non -irradiated parents.Female-biased sex ratios were observed at all treatments in whether parental or F1 generation..The data indicated that the host eggs of the F1 generation of irradiatedE. Calidella was less effective for parasitization by T. evanescens females than the host eggs descendant of the parents and the control treatments. These results could lead to maximize the integrated E. calidella control with gamma irradiation and T. evanescens parasitism.

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