Laboratory bioassay of some entomopathogenic fungi on Spodoptera littoralis (Boisd.) and Agrotis ipsilon (Hufn.) larvae (Lepidoptera: Noctuidae).

Document Type : Original Article

Authors

Pests and Plant Protection Department, National Research Centre, El-Tahrir St., Dokki, Cairo, Egypt

Abstract

        Efficacy of entomopathogenic fungi products (i.e. Bio- Power (Beauveria bassiana), Bio-Catch (Lecanicillium lecanii) and Priority (Paecilomyces fumosoroseus)) against Spodoptera littoralis and Agrotis ipsilon larvae were evaluated under laboratory conditions. Four different concentrations i.e. 0.125x109, 0.25x109, 0.5x109 and 1x109 spores/ 1000ml D.W of each formulation were used against each pest under investigation and compared with control insects. The results obtained show that Bio- Power was the most effective product followed by Bio-Catch and Priority against S. littoralis 3rd instar larvae, whereas , the LC50,90 values were 0.2x109 and 1.5x109, 0.22x109 and 4.6x109 , 0.44x109 and 4.7x109  , respectively. While in case of A. ipsilon, Priority was the most potent product followed by Bio- Power and Bio-Catch, whereas, the LC50,90 values were 0.14x109 and 0.6x109, 0.2x109 and 0.9x109 , 0.4x109 and 9.7x109 , respectively. Also, The results obtained show that percentage mortalities of  treated third instar larvae of S. littoralis with Bio- Power, Bio-Catch and Priority were 87.5,72.5 and 67.5 %, respectively ,within 6.3,8.2 and 7.4 days at the highest concentration used (1x109 spores). However, The treated third instar larvae of A. ipsilon with Priority, Bio- Power and Bio-Catch achieved 100, 90.0 and 62.5 % mortality, respectively, within 5.3, 7.1and 3.9 days at concentration of 1x109 spores. The different formulations had delayed effects, the percentage of pupae and adults emerged were significantly decreased with an increase in concentrations.

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