Follow Up Effectiveness for A Field Application of a Chlorfluazuron Compound (Topron) On Long-Term on Earias insulana (Boisd.) Through the Histological Examination of The Integument

Adly, Ayman, M.

doi:10.21608/eajbsf.2020.107139

Follow Up Effectiveness for A Field Application of a Chlorfluazuron Compound (Topron) On Long-Term on Earias insulana (Boisd.) Through the Histological Examination of The Integument

Document Type : Original Article

Author

Ayman, M. Adly

Plant Protection Research Institute, Dokki, Giza, Egypt

10.21608/eajbsf.2020.107139

Abstract

Under a single field application, the long-term effect of a chitin synthesis inhibitor, Chlorfluazuron (Topron 5% EC) on spiny bollworm, Earias insulana (Boisd.) was evaluated through histological investigation on the integument as it an essential background to the action of that insecticide. After two weeks off use, samples of large-sized larvae (12 - 15 days age-old approximately) under the effect of the procedure were to represent the criterion of the long-term for the tested compound. The microscopic examination of the treated sections showed distinct changes in the tissue components of integument compared to the untreated specimens. The treated sections showed an increase in the thickness of the epidermis layer with noting that there is no new layer of cuticle or show any other signs of further development. Various specimens of the treated larval sections revealed the presence of an individual layer of cuticle, which looked thick, and the spines were rough, extra-long, and tanned. Also, in many sections, the cuticle had been seen shrinkage from the epidermis. The current study confirms and suggests that the long-term impact must be considered for such compounds when evaluated under field conditions.

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