ORIGINAL_ARTICLE
Effect of some natural products on the vector of Bancroftian filariasis in Jizan, KSA
Rotenone, limonine and jojoba oil bioactivity was studied on the 3rd instar larvae of Culex (Cx.) pipiens pallens as a Bancroftian filariasis vector and the prevalent mosquito in Jizan, KSA. They induced toxic effects on different stages of Cx. pipiens, but in different percent. They revealed Lc50of 13.6, 658.39 and 5991.5 ppm for rotenone, limonine and jojoba oil respectively.
https://eajbsf.journals.ekb.eg/article_17015_86302d66a2f4976dbf95f8e43b9d621d.pdf
2018-06-01
1
11
10.21608/eajbsf.2018.17015
Rotenone
limonine and jojoba oil
of Culex pipiens
Jizan
KSA
Reda
Bakr
redabakr55@gmail.com
1
Department of Entomology, Faculty of Science, Ain Shams University, Egypt-Biology Department, Faculty of Science and arts, Baisha University, Baisha
AUTHOR
Mamdouh
Nassar
2
Department of Entomology, Faculty of Science, Cairo University, Cairo, Egypt
AUTHOR
Nehad
El-Barky
3
Department of Entomology, Faculty of Science, Benha University, Al Qalyubia, Egypt
AUTHOR
Mohammed
Abdeldayem
4
-Department of Biology, Faculty of Science, Jazan University, Jazan, KSA - Virology Sector, VACSERA – Egypt
AUTHOR
Thorayia
Kotb
5
Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt
AUTHOR
ORIGINAL_ARTICLE
Enzymatic Changes and Toxic Effect of Some Aromatic Plant Oils on The Cotton Leafworm, Spodoptera littoralis (Boisd.)
In order toevaluate the larvicidal and biochemical effects of some aromatic plant oilsagainst the cotton leafworm larvae, Spodoptera littoralis (Boisduval),the present investigation was carried out. Four commercial aromatic oils;garlic, mint, eucalyptus, and lavender oils, were tested against 2ndand 4th instar larvae. Results showed that all tested oils were soefficient, especially; the garlic oil as it exhibited the least LC50value for both 2nd and 4th instar larvae. Results alsorevealed significant disruption (either reduced or enhanced) enzyme activity.In conclusion, tested oils proved to have insecticidal, antifeedant, andinhibitory enzymatic activity effects. So that, tested oils can be appliedsafely in IPM program for the cotton leafworm, Spodoptera littoraliscontrol.
https://eajbsf.journals.ekb.eg/article_17016_5fce786b8af661d780be0f4260b9ee7b.pdf
2018-06-01
13
24
10.21608/eajbsf.2018.17016
Aromatic plant oils
Cotton leafworm
larvicidal effect
detoxification enzymes
non-specific esterases
Acid and alkaline phosphatases
Suzan
Ibrahim
1
Cotton Leafworm Research Department, Plant Protection Research Institute, Dokki, Giza, Egypt
AUTHOR
Sara
Abd El-Kareem
saraelkhateeb148@gmail.com
2
Cotton Leafworm Research Department, Plant Protection Research Institute, Dokki, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Effects of Two Neonicotinoids Insecticides on Some Anti-Oxidant Enzymes and Hematological Parameters in Egyptian Frogs, Bufo regularis.
The effect of two neonicotinoid pesticides,Actra and Acetamore, on the Egyptian frog (Buforegularis) was evaluated. Some parameters were selected to reflect andmeasure the stress caused by exposing frogs to 1/6 of the recommended fieldconcentration. Stress parameters included malondialdehyde (MDA), theantioxidant enzyme, reduced glutathione (GSH) and superoxide dismutase (SOD)besides some hematological parameters. Results showed that activities of the enzymes GSH and SOD decreasedsignificantly after treatments with both pesticides. GSH content decreased by29.47% and 47.37% compared to control frogs, while SOD activity decreased by11.37% and 18.88% after exposure to Actra and Acetamore pesticides,respectively. The exposure for both pesticides promotes significant increasein MDA content and also caused asignificant reduction for serum albumin fraction and total proteins. The numberof total red blood cells (RBCs) and hematocrit values showed a non-significantdecrease, whereas the number of white blood cells (WBCs) increasedsignificantly by 82.54% and 127.5%, after the exposure to Actra and Acetamore,respectively. Hemoglobin content and the blood indices such as Mean corpuscularvolume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular HemoglobinConcentration (MCHC) declined significantly only in Acetamore treated frogs.Changes caused by Acetamore exposure were much more profound for nearly allstudied parameters in comparison to changes caused by Actra. Alteration tookplace in the studied parameters could have adverse influence on major physiologicalfunctions in frogs, which could result in more vulnerable adults.
https://eajbsf.journals.ekb.eg/article_17017_b5d5e140d5503be890246182fefe0ffa.pdf
2018-06-01
25
36
10.21608/eajbsf.2018.17017
Frogs
Antioxidant enzymes
hematology
neonicotinoids pesticides
Naglaa
Loutfy
naglamag@yahoo.com
1
Department of Plant Protection, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt
AUTHOR
Marwa
Kamel
2
Department of Plant Protection, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt
AUTHOR
ORIGINAL_ARTICLE
Appraisal of Positive Pesticides Influence on Pink Bollworm Larvae, Pectinophora gossypiella (Saunders)
Experimentsconducted to study the toxic and biological effects of three chemicalinsecticides (chlorpyrifos, Lambda-Cyhalothrin, and Methomyl), onebiochemical insecticide (spinosad), and two bio-insecticides (Bacillusthuringiensis kurstaki Bt and Nucleopolyhedrovirus, NPV) against neonatelarvae of the pink bollworm, Pectinophora gossypiella (Saund.), were carried out underlaboratory conditions. The highest effect of tested pesticides on larvalmortalities was recorded after four days of feeding these larvae on treateddiet. Spinosad was the effective toxin (LC50=1.84 ppm) to neonatelarvae followed by chlorpyrifos (LC50= 3.06 ppm), Lambda-Cyhalothrin(LC50= 5.99 ppm) then Methomyl (LC50=23.50 ppm) but B.t.(74.84×104 I.U) and NPV (83.5×109 I.U) were the lowestones. LC50 treatments of neonate larvae with all tested insecticidesproduced reduction in larval and pupal weights especially Lambda-cyhalothrintreatment, (31.9 and 24.3 %, resp.) compared with untreated insects. Thedecreasing effect of all treatments observed in fecundity and life span ofadult females which reflected in very high percentages of insect sterility withchlorpyrifos (66.7%) and Methomyl (61%) treatments. Insignificant increase oftotal protein contents and activity of Glutathione-S-transferase enzyme wasdetected in tissues of LC50 treated larvae compared with untreated larvae,but a significant (23.5%) and highly significant (56.9%) increase in enzymeactivity were resulted by Chlorpyrifos and Spinosad treatments. A very highinhibition in activity of Acetylcholinesterase enzyme of larval tissues wasrecorded with NPV (61.3%), B.t. (58.8%), Lambda-Cyhalothrin (55.6%), Spinosad(54.4%), Chlorpyrifos (48.7%), and Methomyl (37.5%)treatments than untreated ones.
https://eajbsf.journals.ekb.eg/article_17018_005381f30cbf5bc91883c031bba68794.pdf
2018-06-01
37
47
10.21608/eajbsf.2018.17018
pink bollworm
Chemical pesticides
biochemical insecticides
bio-insecticides
biological aspects
GST and AchE enzymes
Eman
Radwan
1
Agricultural Research Center, Central Agricultural Pesticides Laboratory, Ministry of Agriculture, Dokki, Giza
AUTHOR
Mohamed
El-Malla
2
Agricultural Research Center, Central Agricultural Pesticides Laboratory, Ministry of Agriculture, Dokki, Giza
AUTHOR
Mohamed
Fouda
3
Faculty of Science, Department of Zoology, Al-Azhar University, Nasr City, Cairo, Egypt
AUTHOR
Ragab
Mesbah
amragabdallah@gmail.com
4
Agricultural Research Center, Central Agricultural Pesticides Laboratory, Ministry of Agriculture, Dokki, Giza
AUTHOR
ORIGINAL_ARTICLE
Role of Teflubenzuron as A Chitin Synthesis Inhibitor Against Spodoptera littoralis Larvae
The present research focuses on the effect of teflubenzuron on the 4th larval instar of the cotton leafworm, Spodoptera littoralis under laboratory conditions. Teflubenzuron exhibited larvicidal activity (LC50 8.45 ppm) after 72 hours of treatment. Scanning electron microscopy (SEM) showed growth disruption and abnormalities in the external morphological features of cuticle and head capsule in larvae treated with teflubenzuron. Marked biochemical changes in chitin synthesis pathway were recorded. Protease enzyme activity was significantly reduced, whereas the activities of both chitinase and phenoloxidase were significantly elevated in treated larvae in comparison to controls.
https://eajbsf.journals.ekb.eg/article_17019_c5c2b4b92531a059f5bd83ce19fa02fb.pdf
2018-06-01
49
58
10.21608/eajbsf.2018.17019
Teflubenzuron
Spodoptera littoralis
Chitin synthesis inhibitors
Enzymes
Ultrastructure
Hala
Mead
1
Plant Protection Research Institute, Agricultural Research Center, Doqqi, Giza, Egypt
AUTHOR
Mohamad
Khedr
m3khedr@yahoo.com
2
Plant Protection Research Institute, Agricultural Research Center, Doqqi, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Evaluation of Entomopathogenic Fungi, Beauveriabassiana And Metarhizium anisopliae on Peach Fruit Fly, Bactrocera zonata (Saunders) (Diptera:Tephritidae)
This research intended to investigate thepathogenicity of Beauveria bassiana (Bals.) and Metarhiziumanisopliae (Met.) on immature stages and adult flies of the peach fruit fly, Bactrocerazonata (Saunders). Percentage mortality was concentration dependent.The LC50 and LC90 values reflected privilege of B.bassiana over M. anisopliae on immature stages while B. bassianareflected higher mortality values for young age pupae than those of old age.Both entomopathogenic fungi showed high activity against flies.
https://eajbsf.journals.ekb.eg/article_17020_ba944fff06e0655f96418549e708b238.pdf
2018-06-01
59
68
10.21608/eajbsf.2018.17020
Bactrocera zonata
Beauveria bassiana
Metarhizium anisopliae
Biocontrol
Mohamed
hussein
1
Faculty of Science-Ain Shams University, Cairo, Egypt
AUTHOR
Amany
Khaled
2
Faculty of Science-Ain Shams University, Cairo, Egypt
AUTHOR
A.
Ibrahim
3
Plant Protection Research Institute-Agricultural Research Center, Dokki, Giza, Egypt.
AUTHOR
Nehad
Soliman
4
Plant Protection Research Institute-Agricultural Research Center, Dokki, Giza, Egypt.
AUTHOR
Safaa
Attia
5
Plant Protection Research Institute-Agricultural Research Center, Dokki, Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Residuals Efficacy of Thiamethoxam and Thiaclopride Soil Applications against Whitefly, Bemisia tabaci (Homoptera; Aleyrodidae) and Their Impact on Soil Microarthropods
Field studies werecarried out to investigate the efficacy of thiamethoxam (TMX) and thiaclopride(THI) via three different types of soil against whitefly, Bemisia tabaci,on tomato seedlings during two successive seasons. Physical and chemicalcriteria were determined in each of soil type. Results revealed that, the soiltypes were loamy sand (Al-Maamoura), silt loam (Bangar Al-Sokkar) and clay(Abou-Homous). During 2016 and 2017, clay soil had the highest contents inorganic matter (3.43 and 3.48% respectively) and organic carbon (1.99 and 2.03%respectively). The highest mean reduction percentages of whiteflytreatedwith the TMX in loamy sand soil were 79.35 and 80.12% and in silt loam were78.39 and 81.60% during 2016 and 2017comparing to clay soil. Likewise, THI hadthe highest mean reduction percentages during 2016 and 2017 in loamy sand soil (78.06and 79.88% respectively) and silt loam (77.12 and 78.56% respectively) comparedto clay soil. Residuals efficacy of TMX and THI extended to 15 days in loamysand and silt loam soils compared to 9 days in clay soil during the two seasonsand the season of 2016 respectively. During the season of 2017, THI had moreextension for a week in the three tested soils. Initial effects againstwhitefly had a rapid onset on the 3rd day post-treatments of TMX inloamy sand and THI in silt loam and clay soils during the two seasons. Initialeffects delayed on the 9th day post treatment of TMX in silt loamsoil during season of 2017and in clay soil during the two seasons.Retardations, extended to the 9th day of THI treatment in loamy sandsoil during the season of 2017. The efficiencies of TMX and THI via soils ofloamy sand and silt loam against whitefly were significantly more than claysoil. Full recovery levels ofmicro-arthropods revealed at the end of the 7th week in all soil’s depths of loamy sand and siltloam treated with TMX and in loamy sand soil treatedwith THI during the two seasons. Treated areas of high OM -clay soil failed to reach the recovery levels.
https://eajbsf.journals.ekb.eg/article_17021_f64c2e648e0bf0775ab769be9fd5b1c9.pdf
2018-06-01
69
84
10.21608/eajbsf.2018.17021
Residuals efficacy
thiamethoxam
thiaclopride
Bemisia tabaci
micro-arthropods
Wael
Khamis
wmkhamis74@yahoo.com
1
Dept. of Cotton Pesticides Bioassay Research, Plant Protection Research Institute, Agricultural Research Center, Al Sabhia, Alexandria, Egypt
AUTHOR
ORIGINAL_ARTICLE
Nematicidal Potential Impact of Phosphodiesterase (PDE) Inhibitors against Root-Knot Nematode, Meloidogyne incognita.
Soon after the discovery that phosphodiesterase (PDEs) are presented in nematodes and regulate the signaling pathway responsible for its motility and reproduction, many of PDE inhibitors have been examined for their nematicidal effects. The present study evaluates the nematicidal activity of seven commercial drugs that act as PDE inhibitors namely diazepam, papaverine, theophylline, caffeine, cilostazol, vinpocetine and sildenafil against root-knot nematodes (Meloidogyne incognita) under laboratory and greenhouse conditions. The results showed that all tested PDE inhibitors reduced tomato root galling significantly compared with the untreated check. Diazepam, papaverine and caffeine treatments gave the highest reduction in root galls by 95.5, 93.3 and 91%, respectively.Diazepam exhibited strong nematicidal activity against second-stage juveniles (J2) of M. incognita with 48hrs-LC50 value of 4.63 ppm. Therefore, using phosphodiesterase inhibitors could serve as the basis for novel nematicides with greater efficacy and reduced environmental impact.
https://eajbsf.journals.ekb.eg/article_17022_e21425bec3b11511d4f56bc3a31614b7.pdf
2018-06-01
85
90
10.21608/eajbsf.2018.17022
Root-knot nematodes
Meloidogyne incognita
Phosphodiesterase (PDE)
PDE inhibitors
Raddy
M.
hmraddy@azhar.edu.eg
1
Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Egypt
AUTHOR
Abdel-Lateef
F.
2
Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Egypt
AUTHOR
Fouad
Ali
3
Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Egypt
AUTHOR
Montasser
A.
4
Department of Agricultural Zoology and Nematology, Faculty of Agriculture, Al-Azhar University, Egypt
AUTHOR