Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
2090-0791
2090-0864
4
1
2012
12
01
Toxic and biochemical effects of different insecticides on the tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)
1
10
EN
Eman
M. M.
Radwan
Central Agricultural Pesticides Laboratory, Agricultural Research Center Giza-Egypt
Hanna
S.
Taha
Central Agricultural Pesticides Laboratory, Agricultural Research Center Giza-Egypt
10.21608/eajbsf.2012.17272
Toxic effect of dinotefuran, imidacloprid, fenoxycarb, phenthoate and thiocyclam H.O insecticides on greenhouses population of the tomato leafminer,<em>Tuta absoluta</em> (Meyrick) was evaluated in laboratory. Data revealed that the five tested insecticides had high contact toxic effect on moths and 3<sup>rd</sup> instar larvae of theinsect. Moths were more susceptible to the effect of tested insecticides than larvae. Imidacloprid was the superior toxicant against moths and larvae, so it had a very low resistance coefficient (RC= 0.01 and 0.13). Phenthoate and thiocy- clam-H.O. had high toxic effect on two stages. Dinotefuran seemed to have low effect on moths and the same trend was observed with fenoxycarb on larvae. The activity of acetycholinesterase (AChE), glutathione-S-transferase (GST) and monooxygenase (PCMAN-demethylase) was higher in whole body homogenate of untreated moths (1.5, 1.2 and 1.58 times, respectively), than that of 3<sup>rd</sup> instar larvae. The exposure of moths and 3<sup>rd</sup> instar larvae to LC<sub>30</sub>, LC<sub>50</sub> and LC<sub>80 </sub>of tested insecticides caused significant reduction (51.11and 25.00%) or increasing (41.78 and 28.77%) in activity of AChE, respectively. Phenthoate treatments reduced AChE activity, but imidacloprid and dinotefuran induced this activity in LC<sub>80</sub> treated insects .Low insecticide treatments produced slight induction (1.41-11.90%) of GST, other two treatments produced reduction or increasing enzyme activity of treated insects. LC<sub>80 </sub>of fenoxycarb had moderate induction (24.79- 27.90%) of moths and larvae GST. A positive correlation between the insecticide concentration and the activity of monooxygenase PCMAN-demethylase was observed in treated insects with five insecticides. Elevation of enzyme activity ranged from 1.59 to 52.12% in moths and 5.83- 59.17% in larvae. Phenthoate and imidacloprid produced the higher induction effect of treated insect enzyme than the other three insecticides.
Tuta absoluta,Insecticides,Acetycholinesterase,glutathione-S-transferase,PCMAN-demethylase Monooxygenase
https://eajbsf.journals.ekb.eg/article_17272.html
https://eajbsf.journals.ekb.eg/article_17272_015bb94adb454c2aa774d3e83c564cd8.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
2090-0791
2090-0864
4
1
2012
12
01
Biological and behavioral effects of Pyriproxyfen on pheromone production and perception. of Tribolium castaneum (Coleoptera: Tenebrionidae).
11
22
EN
Nehad
M.
El-barky
Entomology Department, Faculty of Science, Benha University, Qalyubiya, Egypt
Olfat
M.
El-Monairy
Entomology Department, Faculty of Science, Benha University, Qalyubiya, Egypt
Reda
F. A.
Bakr
Entomology Department, Faculty of Science, Benha University, Qalyubiya, Egypt
Nancy
M. B.
El-shourbagy
Entomology Department, Faculty of Science, Benha University, Qalyubiya, Egypt
10.21608/eajbsf.2012.17274
Pyriproxyfen (Admiral) is an insect growth regulator (IGR) acts as anti- juvenile hormone. The present work aims to investigate the toxicological effect of Pyriproxyfen on both sexes of rust red flour beetle, <em>Tribolium castaneum</em> resulted from treated 4<sup>th</sup> larval instar with LC<sub>50</sub> value (2.4ppm) and its effect on female production and male's perception to pheromone. The results indicated that both treated and untreated sexes could secrete a pheromone that was able to stimulate the opposite sex as well as its own sex. But production and responsiveness of pheromone in untreated groups were significantly higher than treated one.
Females secreted a pheromone is a sex pheromone, While the pheromone secreted by males is an aggregation pheromone
Tribolium castaneum,Pyriproxyfen,pheromone production and perception,daytime,Age,Hunger,temperature and antennal sensillae
https://eajbsf.journals.ekb.eg/article_17274.html
https://eajbsf.journals.ekb.eg/article_17274_a502c0c980a79735f25a799b08d6f3d2.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
2090-0791
2090-0864
4
1
2012
12
01
Effect of katel-sous dust and clove powder and their mixtures on the cowpea seed beetle, Callosobruchus maculatus (F.) (Coleoptera: bruchidae)
23
33
EN
Rasha
F.
Sokker
Plant Protection Research Institute, Stored Products Department, Agriculture Research Center
Mohamed
A.
Hussein
Faculty of science, Entomology Department, Ain Shams University
Salwa
M. S.
Ahmed
Plant Protection Research Institute, Stored Products Department, Agriculture Research Center
Ragaa
K. A.
Hamed
Faculty of science, Entomology Department, Ain Shams University
10.21608/eajbsf.2012.17277
<span style="font-family: 'Times New Roman','serif'; font-size: 12pt; mso-fareast-font-family: Calibri; mso-fareast-language: EN-CA; mso-bidi-language: AR-SA; mso-ansi-language: EN-CA;" lang="EN-CA">The present work was<br />carried out to evaluate the toxicity of katel-sous dust and clove powder and<br />its combinations. </span><span style="font-family: 'Times New Roman','serif'; font-size: 12pt; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: EN-CA; mso-bidi-language: AR-SA; mso-ansi-language: EN-CA; mso-bidi-font-weight: bold;" lang="EN-CA">Evaluation has been conducted on <em>Callosobruchus maculatus</em> (F.)<br />adults to determine the toxicological effect and the changes in insect biology<br />caused by these products. The results referred to the availability to use these<br />products to control cowpea seed beetle and consider clove powder as<br />spiracle-blocking insecticides. Clove powder and katel-sous dust and its<br />combinations made a sharp reduction in the number of eggs laid and the number<br />of adults emerged. Mixing clove powder with katel-sous dust was helping in<br />using katel-sous dust in pest management without any problems such as; its high<br />concentration which caused suffering from the expensive costs and hardly remove<br />the dust.</span>
Katel-sous,Clove powder,control,Bruchids,Coleoptera,Callosobruchus maculatus,Cowpea seed beetle and Scanning
https://eajbsf.journals.ekb.eg/article_17277.html
https://eajbsf.journals.ekb.eg/article_17277_b7f108552f1a6c7653914b541077b07a.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
2090-0791
2090-0864
4
1
2012
12
01
Toxicological and biochemical studies of lufenuron, chlorfluazuron and chromafenozide against Pectinophora gossypiella (Saunders)
37
47
EN
Mrvat
A.
kandi
Plant protection research inistitute, A.R.C., Dokki-Giza, Egypt
A.
F.
Ahmed
Plant protection research inistitute, A.R.C., Dokki-Giza, Egypt
Hemat
Z.
Moustafa
Plant protection research inistitute, A.R.C., Dokki-Giza, Egypt
10.21608/eajbsf.2012.17281
In the present study, one day old eggs laboratory colony of <em>Pectinophora gossypiella</em>, were treated with estimated LC<sub>50</sub> values of lufenuron 5%, chlorfluazuron 5% and chromafenozide 80% (3.471, 4.189 and 122.703 ppm respectively), to study their effects on percentage of hatchability and duration of subsequent larval, pupal, immature stages and longevity, fecundity and fertility of resulted adults. The obtained results clear that the percentage of hatchability of treated eggs were 49.6, 51.0 and 53.0 for the three tested compounds, respectively oppose to 97% in untreated control. The incubation period of the hatched eggs was 6.5, 4.7, 6.2 and 3.2 days at three (IGR<sub>S</sub>) and control, respectively. Also, the treatments affected the subsequent stages it caused significant effects on larval and pupal duration, weight and malformations. On the other hand, the adult stage resulted from treated eggs was highly affected by the three (IGR<sub>S</sub>). All the compounds especially chlorfluazuron 5% caused increasing in adult longevity and reduced the respective fecundity and fertility compared to control. The biochemical effects of the tested insect growth regulators as chitin synthesis inhibitor (CSI) against larvae resulted from (treated one-day old eggs) was studied. The obtained results indicated that the tested IGR<sub>S</sub> reduced the glucose, protein and carbohydrate contents. Also, the tested IGR<sub>S</sub>elicited inhibitory effect on alanine amino transferase (ALT) and aspirate amino transferase (AST).
Pectinophora gossypiella,PBW,IGRs,toxicity,LC50,biological studies,Biochemical analysis
https://eajbsf.journals.ekb.eg/article_17281.html
https://eajbsf.journals.ekb.eg/article_17281_299bb047703074fcf190ee668facb1d8.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
2090-0791
2090-0864
4
1
2012
12
01
Effect of three insect growth regulators (Chlorfluazuron, Tebofenozoid and pyriproxyfen) on fecundity of S. littoralis, histopathological and some biochemical aspects of moth ovary.
49
59
EN
Aziza
E.
Abdel-Aal
Plant Protection Research Institute, A.R.C., Dokki-Giza, Egypt
10.21608/eajbsf.2012.17282
The insecticidal and biological effects of three insect growth regulator (chlorfluazuron, Tebofenozoid and pyriproxyfen) were evaluated on 4<sup>th </sup> instar larvae of <em>Spodoptera littoralis</em> (Boisd.) (Lepidoptera: Noctuidae). Based on the LC<sub>50</sub> values Chorofluazuron is the more toxic to <em>S. littoralis</em> than that of the two other compounds. Female longevity, fecundity and fertility were significantly reduced at all treatments compared to control. Furthermore, different levels of significant changes in the total protein, carbohydrate contents of the female ovaries pre-treated as 4<sup>th</sup> instar larvae by LC<sub>50 </sub>of used IGRs were recorded. Moreover, different abnormal histological structures of ovary were noticed.
Insect growth regulators (IGR's),toxicity,LC50,Spodoptera littoralis,biological,biochemical and histological aspects
https://eajbsf.journals.ekb.eg/article_17282.html
https://eajbsf.journals.ekb.eg/article_17282_3febf8f03fea77ae403a421316ddf6bf.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
2090-0791
2090-0864
4
1
2012
12
01
Efficiency of Diple 2x ((Bacillus thuringiensis var. kurstaki) alone and its mixture with two insecticides against the Egyptian cotton leaf worm Spodoptera littoralis (Lepidoptera: Noctuidae).
61
68
EN
Aziza
E.
Abdel-Aal
Plant Prot. Res. Inst., Agric. Res. Cent., Dokki, Giza, Egypt
El- Shikh
T.
A.
Plant Prot. Res. Inst., Agric. Res. Cent., Dokki, Giza, Egypt
10.21608/eajbsf.2012.17285
The Efficiency of the bioinsecticide, Diple 2x (<em>Bacillus thuringiensis</em>) alone and its mixtures with two insecticides on cotton leafworm <em>Spodoptera littoralis </em>(Boisd) was investigated. Data indicated that Lufenuron was the highest effective insecticides against both the 2<sup>nd</sup> and the 4<sup>th</sup> instar larvae of<em> S</em>.<em> littoralis</em> followed by Methomyl and B.t , respectively .All tested mixtures decreased the toxicity according to Co-Toxicity factor ,the tested mixture (B.t + Lufenuron) gave the high level of antagonism effect followed by the mixture (B.t + Methomyle). The field efficiency of tested insecticides (when used singular) can be arranged according to the general mean of reduction percentage during two seasons in a descending order as follows: Lufenuron, Methomyle and B.t. The addition of B.t. to Lufenuron gave raising the general mean of reduction percentage during two seasons in contrast the other tested insecticide gave a reduction but less than when applied without added to Bt. The same trend of effects were obtained when applied the two tested insecticides at the half recommended rate alone or mixed with the same dose of Bt on<em> S. littoralis</em> but with less level of reduction in case of (Bt+Lufenuron) mixture.Also the three toxicants affected the Chitinase ,prptease and acetycholinesterase enzymes.
Bacillus thuringiensis var,Spodoptera littoralis
https://eajbsf.journals.ekb.eg/article_17285.html
https://eajbsf.journals.ekb.eg/article_17285_b6b97a3b678061a3c283d3d29528cf4d.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
2090-0791
2090-0864
4
1
2012
12
01
The efficiency of different materials for controlling vine mealybug, Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae)on grapes during the period of investigation.
69
75
EN
Mohamed
G.
H.
Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt
Suzan
A.
Badr
Plant Protection Research Institute, Agriculture Research Center, Sabahia, Alexandria, Egypt
S.
M.
El-Amir
Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt
10.21608/eajbsf.2012.17286
<strong> </strong>
The vine mealybug, <em>Planococcus ficus</em> (Signoret) (Hemiptera: Pseudococcidae) was a majour pest in many grape vines growing regions in the world and Egypt. It is feeding on several agricultural and weedy plant species. However, grape vines are a preferred host, and are the most adversely affected by this insect. Like other mealybugs, vine mealybug feeds on phloem sap, but it can be found throughout the plant-even on the roots. The pest were suppressed by preventive measures with a view to reducing the impact of the attack. Allowed substances acting on the suitable time, if appropriate. Insecticide of plant origin citric acid are used in May before coloration. Along with the biological control of <em>Chrysoperla carnea </em>in July after coloration, as well as, low toxicity insecticide (Malathion) in January after pruning process. In the spring where the small buds appear a sequence of mineral oil (KZ) are used. The obtained data showed a significant effect on the different stages of the total insect population The obtained data indicated that, tested compound Citric acid on <em>P. ficus</em> infested grape showed a slight effect after three days from treatment, then their effect increased gradually to give the highest effectiveness after 18 days. Using the predator, <em>C. carnea</em> for controlling <em>P. ficus</em> infested grape, showed the effectiveness of this predator in reducing the <em>P. ficus</em> after releasing.
vine mealybug,Planococcus ficus
https://eajbsf.journals.ekb.eg/article_17286.html
https://eajbsf.journals.ekb.eg/article_17286_a2cfb95a4046b210d5f4eec209c1efd9.pdf